Capillary driven microfluidic sequential flow device for point-of-need ELISA: COVID-19 serology testing

Author:

Carrell Cody1,Jang Ilhoon12ORCID,Link Jeremy1,Terry James S.3,Call Zachary1,Panraksa Yosita4,Chailapakul Orawon4,Dandy David S.56ORCID,Geiss Brian J.36ORCID,Henry Charles S.156ORCID

Affiliation:

1. Department of Chemistry, Colorado State University, CO, 80523, USA

2. Department of Mechanical Engineering, Hanyang University, Seoul, 04763, Korea

3. Department of Microbiology, Immunology and Pathology, Colorado State University, CO, 80523, USA

4. Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

5. Department of Chemical and Biological Engineering, Colorado State University, CO, 80523, USA

6. School of Biomedical Engineering, Colorado State University, CO, 80523, USA

Abstract

A capillary-driven microfluidic sequential flow device, designed for eventual at-home or doctor's office use, was developed to perform an enzyme-linked immunosorbent assay (ELISA) for serology assays.

Funder

National Research Foundation of Korea

National Institutes of Health

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Reference26 articles.

1. Comparison of 2 fully automated tests detecting antibodies against nucleocapsid N and spike S1/S2 proteins in COVID-19

2. Current advances in the detection of COVID-19 and evaluation of the humoral response

3. Q. Diagnostics , COVID-19 test price information , https://www.questdiagnostics.com/business-solutions/health-plans/covid-19/pricing , accessed March 13, 2022

4. COVID-19 Target product profiles for priority diagnostics to support response to the COVID-19 pandemic v.0.1 , World Health Organization , 2020

5. Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

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