Detection of CpG methylation level using methyl-CpG-binding domain-fused fluorescent protein-Reference-Cited by-同舟云学术

Detection of CpG methylation level using methyl-CpG-binding domain-fused fluorescent protein

Published:2023 Issue:19 Volume:15 Page:2294-2299
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Fujita Marika¹,Goto Masanori¹,Tanaka Masayoshi²,Yoshida Wataru¹³^ORCID

Affiliation:

1. Graduate School of Bionics, Tokyo University of Technology, 1404-1 Katakuramachi, Hachioji, Tokyo, 192-0982, Japan

2. Department of Chemical Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8503, Japan

3. School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakuramachi, Hachioji, Tokyo, 192-0982, Japan

Abstract

We developed a hybridization-based CpG methylation level detection system using methyl-CpG binding domain (MBD)-fused fluorescent protein, which is applicable to genome-wide DNA modification detection using microarrays.

Funder

Murata Science Foundation

Cosmetology Research Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AY/D3AY00227F

Reference43 articles.

1. Occurrence of 5-Methyl-Cytosine in Nucleic Acids

2. THE ACTION OF PANCREATIC DESOXYRIBONUCLEASE

3. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality

4. DNA Methyltransferases Dnmt3a and Dnmt3b Are Essential for De Novo Methylation and Mammalian Development

5. CpG islands as gene markers in the human genome

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