Fabrication of 3D continuous-flow reverse-transcription polymerase chain reaction microdevice integrated with on-chip fluorescence detection for semi-quantitative assessment of gene expression-Reference-Cited by-同舟云学术

Fabrication of 3D continuous-flow reverse-transcription polymerase chain reaction microdevice integrated with on-chip fluorescence detection for semi-quantitative assessment of gene expression

Published:2018 Issue:23 Volume:143 Page:5692-5701
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Pham Quang Nghia¹²³^ORCID,Trinh Kieu The Loan¹²³^ORCID,Tran Nguyen Khoi Song⁴²⁵^ORCID,Park Tae-Sik⁴²⁵^ORCID,Lee Nae Yoon¹²³^ORCID

Affiliation:

1. Department of BioNano Technology

2. Gachon University

3. 13120, Korea

4. Deparment of Life Science

5. Korea

Abstract

A 3D microdevice equipped with a portable pump and a single heater was fabricated integrating RNA amplification and detection functionalities.

Funder

National Research Foundation of Korea (NRF)

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2018/AN/C8AN01739E

Reference45 articles.

1. Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells

2. Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors

3. Integrated RNA extraction and RT-PCR for semi-quantitative gene expression studies on a microfluidic device

4. Rapid and Sensitive Detection of RNA Viruses Based on Reverse Transcription Loop-Mediated Isothermal Amplification, Magnetic Nanoparticles, and Chemiluminescence

5. Integrated miRNA and mRNA expression profiling in response to eriodictyol in human endothelial cells

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