Inducing high activity of a thermophilic enzyme at ambient temperatures by directed evolution-Reference-Cited by-同舟云学术

Inducing high activity of a thermophilic enzyme at ambient temperatures by directed evolution

Published:2017 Issue:68 Volume:53 Page:9454-9457
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Li Guangyue¹²³⁴⁵,Maria-Solano Miguel A.⁶⁷⁸⁹¹⁰,Romero-Rivera Adrian⁶⁷⁸⁹¹⁰,Osuna Sílvia⁶⁷⁸⁹¹⁰^ORCID,Reetz Manfred T.¹²³⁴⁵^ORCID

Affiliation:

1. Max-Planck-Institut für Kohlenforschung

2. Kaiser-Wilhelm-Platz 1

3. Mülheim an der Ruhr

4. Germany

5. Fachbereich Chemie der Philipps-Universität Marburg, Hans-Meerwein-Strasse

6. Institut de Química Computacional i Catàlisi and Department de Química

7. Universitat de Girona

8. Carrer Maria Aurèlia Capmany 6

9. Girona 17003

10. Spain

Abstract

Turn cool off and stay active: the thermostable alcohol dehydrogenase TbSADH originating from the hot springs of Yellow Stone Park was successfully subjected to directed evolution for inducing high activity at ambient temperatures and enabling short reaction times with minimal tradeoff in thermostability. Reversed enantioselectivity was also evolved (99% ee).

Funder

Departament d'Innovació, Universitats i Empresa, Generalitat de Catalunya

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2017/CC/C7CC05377K

Reference63 articles.

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5. Thermozymes: biotechnology and structure-function relationships

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