Test strip coupled Cas12a-assisted signal amplification strategy for sensitive detection of uracil-DNA glycosylase-Reference-Cited by-同舟云学术

Test strip coupled Cas12a-assisted signal amplification strategy for sensitive detection of uracil-DNA glycosylase

Published:2024 Issue:7 Volume:24 Page:1987-1995
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Guo Bin¹,Hu Chong¹,Yang Zeping¹,Tang Chu¹,Zhang Chuanxian²,Wang Fu¹²³^ORCID

Affiliation:

1. Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an 710071, China

2. Institute of Medical Engineering, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an 710061, China

3. Xianyang Key Laboratory of Molecular Imaging and Drug Synthesis, School of Pharmacy, Shaanxi Institute of International Trade & Commerce, Xianyang 712046, China

Abstract

In this study, we employed Cas12a protein and enzyme-assisted cycle amplification technology with a test strip to establish a precise platform for the detection of UDG enzyme.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shaanxi Province

Ministry of Science and Technology of the People's Republic of China

Guangzhou Municipal Science and Technology Project

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/LC/D4LC00096J

Reference32 articles.

1. Nucleotide Excision Repair in Mammalian Cells

2. Role of Base Excision “Repair” Enzymes in Erasing Epigenetic Marks from DNA

3. Base-excision repair of oxidative DNA damage

4. The structural basis of specific base-excision repair by uracil–DNA glycosylase

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