Simultaneous RNA purification and size selection using on-chip isotachophoresis with an ionic spacer

Author:

Han Crystal M.12345ORCID,Catoe David5674,Munro Sarah A.56748,Khnouf Ruba91011121,Snyder Michael P.131474,Santiago Juan G.11574ORCID,Salit Marc L.5674,Cenik Can13147416ORCID

Affiliation:

1. Department of Mechanical Engineering

2. San Jose State University

3. San Jose

4. USA

5. Joint Initiative for Metrology in Biology

6. National Institute of Standards and Technology

7. Stanford

8. Minnesota Supercomputing Institute

9. Department of Biomedical Engineering

10. Jordan University of Science and Technology

11. Irbid

12. Jordan

13. Department of Genetics

14. Stanford University School of Medicine

15. Stanford University

16. Department of Molecular Biosciences

Abstract

We present an on-chip method that achieves simultaneous RNA extraction and size selection, and demonstrate its compatibility with high-throughput sequencing.

Funder

Cancer Prevention and Research Institute of Texas

Material Measurement Laboratory

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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