Low-volume multiplexed proteolytic activity assay and inhibitor analysis through a pico-injector array-Reference-Cited by-同舟云学术

Low-volume multiplexed proteolytic activity assay and inhibitor analysis through a pico-injector array

Published:2015 Issue:4 Volume:15 Page:1153-1159
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Ng Ee Xien¹²³⁴,Miller Miles A.⁵⁶⁷⁸⁹,Jing Tengyang¹²³⁴¹⁰,Lauffenburger Doug A.⁹¹¹¹²⁸,Chen Chia-Hung¹²³⁴¹³

Affiliation:

1. Department of Biomedical Engineering

2. National University of Singapore

3. Singapore 117575

4. Singapore

5. Center for Systems Biology

6. Massachusetts General Hospital

7. Boston

8. USA

9. Department of Biological Engineering

10. Singapore-MIT Alliance for Research and Technology (SMART) Centre

11. Massachusetts Institute of Technology

12. Cambridge

13. Singapore Institute for Neurotechnology (SINAPSE)

Abstract

In this study we developed a pico-injector array to generate 9 × 2 × 2 × 2 = 72 different reactions in picoliter-sized droplets by controlling the sequence of combinational injections, which allows simultaneous read-outs of a wide range of multiple protease reactions and measurement of inhibitor effects by using small sample volumes.

Funder

National University of Singapore

Singapore-MIT Alliance for Research and Technology Centre

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2015/LC/C4LC01162G

Reference35 articles.

1. Quantitative Zymography: Detection of Picogram Quantities of Gelatinases

2. Functional phenotyping of human plasma using a 361-fluorogenic substrate biosensing microarray