Backbone circularization of Bacillus subtilis family 11 xylanase increases its thermostability and its resistance against aggregation-Reference-Cited by-同舟云学术

Backbone circularization of Bacillus subtilis family 11 xylanase increases its thermostability and its resistance against aggregation

Published:2015 Issue:12 Volume:11 Page:3231-3243
ISSN:1742-206X
Container-title:Molecular BioSystems
language:en
Short-container-title:Mol. BioSyst.

Author:

Waldhauer Max C.¹²³,Schmitz Silvan N.¹²³,Ahlmann-Eltze Constantin¹²³,Gleixner Jan G.¹²³,Schmelas Carolin C.¹²³,Huhn Anna G.¹²³,Bunne Charlotte¹²³,Büscher Magdalena¹²³,Horn Max¹²³,Klughammer Nils¹²³,Kreft Jakob¹²³,Schäfer Elisabeth¹²³,Bayer Philipp A.¹²³,Krämer Stephen G.⁴⁵⁶³,Neugebauer Julia⁴⁵⁶³,Wehler Pierre¹²³,Mayer Matthias P.⁷⁸⁶³,Eils Roland¹²³⁴⁵,Di Ventura Barbara¹²³

Affiliation:

1. Center for Quantitative Analysis of Molecular and Cellular Biosystems (BioQuant)

2. University of Heidelberg

3. Germany

4. German Cancer Research Center (DKFZ)

5. Division of Theoretical Bioinformatics

6. Heidelberg

7. Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH)

8. DKFZ-ZMBH-Alliance

Abstract

While using a serine (S) as linker for circularization increases the thermostability, a longer linker (RGKCWE) leads to reduced aggregation after heat shock at elevated temperatures.

Publisher

Royal Society of Chemistry (RSC)

Subject

Molecular Biology,Biotechnology

Link

http://pubs.rsc.org/en/content/articlepdf/2015/MB/C5MB00341E

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