Identification of the over alkylation sites of a protein by IAM in MALDI-TOF/TOF tandem mass spectrometry
Author:
Affiliation:
1. Department of Chemistry
2. Zhejiang University
3. Hangzhou 310027
4. China
5. Key Laboratory of New Drug Research and Clinical Pharmacy
6. Xuzhou Medical College
7. Xuzhou
8. Department of Pharmaceutical Analysis
Abstract
Overalkylation often appears during the proteolytic digestion process when using iodoacetamide (IAM) to protect the produced side chain thiol of Cys from disulfide bonds.
Publisher
Royal Society of Chemistry (RSC)
Subject
General Chemical Engineering,General Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2015/RA/C5RA18595E
Reference33 articles.
1. Competitive formation of b2 and c2-H2O ions from b3 ions containing Asp residue during tandem mass spectrometry: the influence of neighboring Arg
2. The Specific Cleavage of Lactone Linkage to Open-Loop in Cyclic Lipopeptide during Negative ESI Tandem Mass Spectrometry: The Hydrogen Bond Interaction Effect of 4-Ethyl Guaiacol
3. Elimination of Benzene from Protonated N-Benzylindoline: Benzyl Cation/Proton Transfer or Direct Proton Transfer?
4. Matrix-Enhanced Secondary Ion Mass Spectrometry (ME SIMS) Using Room Temperature Ionic Liquid Matrices
5. Imaging of N-Linked Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using MALDI Mass Spectrometry
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