Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML-Reference-Cited by-同舟云学术

Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

Published:2020 Issue:5 Volume:20 Page:942-948
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Yu Jinzhu¹²³⁴,Oh Ki¹²³⁴,Moorthi Sitapriya⁵²⁴,Li Ling¹²³⁴,Strey Helmut H.¹²³⁴⁶,Schuster Michael⁷³⁴,Luberto Chiara⁵²⁴,Quan Phenix-Lan¹²³⁴,Brouzes Eric¹²³⁴⁶^ORCID

Affiliation:

1. Department of Biomedical Engineering

2. Stony Brook University

3. Stony Brook

4. USA

5. Department of Physiology and Biophysics, Renaissance School of Medicine

6. Laufer Center for Physical and Quantitative Biology

7. Hematology Department, Stony Brook Cancer Center

Abstract

We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. This platform may be broadly applied in single or dual parameter assays to study cell population heterogeneity.

Funder

National Cancer Institute

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2020/LC/C9LC01213C

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