Enzyme immobilization on silicate glass through simple adsorption of dendronized polymer–enzyme conjugates for localized enzymatic cascade reactions

Author:

Küchler Andreas12345,Adamcik Jozef67839,Mezzenga Raffaele67839,Schlüter A. Dieter12345,Walde Peter12345

Affiliation:

1. Laboratory of Polymer Chemistry

2. Department of Materials

3. ETH Zürich

4. 8093 Zürich

5. Switzerland

6. Laboratory of Food & Soft Materials

7. Institute of Food, Nutrition and Health

8. Department of Health Sciences and Technology

9. 8092 Zürich

Abstract

Conjugation of enzymes to a dendronized polymer via bis-aryl hydrazone bonds enables simple and stable enzyme immobilisation on unmodified glass.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Reference73 articles.

1. Immobilized enzymes — learning from past successes and failures

2. B. M. Brena and F.Batista-Viera, in Methods in Biotechnology: Immobilization of Enzymes and Cells, ed. J. M. Guisán, Humana Press Inc., Totowa, NJ, 2nd edn, 2006, pp. 15–30

3. Understanding enzyme immobilisation

4. Enzyme Immobilization: The Quest for Optimum Performance

5. Immobilization of enzymes on porous silicas – benefits and challenges

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