β-Terrecyclene synthase constructs the quadrane backbone in terrecyclic acid biosynthesis

Author:

Song Yongxiang1234,Wang Wengui5ORCID,Yang Jiafan123,Gao Dewei4ORCID,Billingsley John M.4,Wang Songtao123,Zhu Yiguang1234,Wang Junfeng1234ORCID,Ju Jianhua13ORCID,Yan Yan1234ORCID,Tang Yi46ORCID

Affiliation:

1. Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China

2. Sanya Institute of Oceanology Eco-Environmental Engineering Yazhou Scientific Bay, Sanya, 572000, China

3. University of Chinese Academy of Science, 19 Yuquan Road, Beijing 100049, China

4. Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, USA

5. School of Chemistry and Chemical Engineering, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, China

6. Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA

Abstract

The construction of a quadrane scaffold by β-terrecyclene synthase via Coates' route is demonstrated through mutagenesis and isotopically sensitive branching studies. Subsequent enzymatic modifications convert β-terrecyclene into terrecyclic acid.

Funder

K. C. Wong Education Foundation

National Key Research and Development Program of China

National Natural Science Foundation of China

Sanya Yazhou Bay Science and Technology City

Guangdong Provincial Pearl River Talents Program

Natural Science Foundation of Hainan Province

Publisher

Royal Society of Chemistry (RSC)

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1. Hot off the press;Natural Product Reports;2024

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