Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases-Reference-Cited by-同舟云学术

Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases

Published:2020 Issue:4 Volume:20 Page:852-861
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Goto Haruna¹²³⁴,Kanai Yuki¹²³⁴,Yotsui Arisa¹²³⁴,Shimokihara Shota¹²³⁴,Shitara Shunya¹²³⁴,Oyobiki Ryo¹²³⁴,Fujiwara Kei¹²³⁴^ORCID,Watanabe Takeshi⁵⁶⁷⁴,Einaga Yasuaki⁸²³⁴^ORCID,Matsumoto Yoshinori⁹²³⁴,Miki Norihisa¹⁰²³⁴,Doi Nobuhide¹²³⁴

Affiliation:

1. Department of Biosciences and Informatics

2. Keio University

3. Yokohama 223-8522

4. Japan

5. Department of Electrical Engineering and Electronics

6. Aoyama Gakuin University

7. Sagamihara 252-5258

8. Department of Chemistry

9. Department of Applied Physics and Physico-Informatics

10. Department of Mechanical Engineering

Abstract

We developed μTAS for screening of NAD(P)-dependent oxidoreductases whose activity can be detected with boron-doped diamond electrodes and whose genes can be recovered by dielectrophoretic sorting of nanoliter-size droplets.

Funder

Asahi Glass Foundation

Japan Society for the Promotion of Science

Ministry of Education, Culture, Sports, Science and Technology

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2020/LC/C9LC01263J

Reference51 articles.

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