An optimised MALDI-TOF assay for phosphatidylcholine-specific phospholipase C-Reference-Cited by-同舟云学术

An optimised MALDI-TOF assay for phosphatidylcholine-specific phospholipase C

Published:2021 Issue:4 Volume:13 Page:491-496
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Sharma Nabangshu¹²³⁴^ORCID,Langley Ries J.⁵²³⁴⁶^ORCID,Eurtivong Chatchakorn⁷⁸⁹¹⁰¹¹^ORCID,Leung Euphemia⁵²³⁴⁶^ORCID,Dixon Ryan Joseph¹²³⁴,Paulin Emily K.¹²³⁴,Rees Shaun W. P.¹²³⁴,Pilkington Lisa I.¹²³⁴^ORCID,Barker David¹²³⁴¹²^ORCID,Reynisson Jóhannes¹²³⁴¹³^ORCID,Leung Ivanhoe K. H.¹²³⁴⁵^ORCID

Affiliation:

1. School of Chemical Sciences

2. The University of Auckland

3. Auckland 1142

4. New Zealand

5. Maurice Wilkins Centre for Molecular Biodiscovery

6. Department of Molecular Medicine and Pathology

7. Program in Chemical Sciences

8. Chulabhorn Graduate Institute

9. Chulabhorn Royal Academy

10. Bangkok 10210

11. Thailand

12. Centre for Green Chemical Science

13. School of Pharmacy and Bioengineering

Abstract

In this manuscript, we report our work in the development and optimisation of a MALDI-TOF mass spectrometry assay to monitor the kinetics and inhibition of PC-PLC, a phospholipase that catalyses the hydrolysis of phosphatidylcholines.

Funder

Royal Society of New Zealand

Cancer Society of New Zealand

Health Research Council of New Zealand

Breast Cancer Foundation New Zealand

University of Auckland

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AY/D0AY02208J

Reference51 articles.

1. D. C. Wilton , in Biochemistry of Lipids, Lipoproteins and Membranes , ed. D. E. Vance and J. E. Vance , Elsevier , Amsterdam , 5th edn, 2007 , ch. 11, pp. 305–329

2. M. Waite , The Phospholipases , in Handbook of Lipid Research 5 , Springer-Verlag , Boston MA , 1987 , ch. 11, pp. 243–281

3. L. A. Hinkel and M. J.Wargo , in Health Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids , ed. H. Goldfine , Springer , Cham , 2020 , pp. 181–203

4. A thraustochytrid-specific lipase/phospholipase with unique positional specificity contributes to microbial competition and fatty acid acquisition from the environment

5. Apparent phosphate retrieval system in Bacillus cereus

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Ultrasensitive electrochemical detection of phospholipase C via dual signal amplification based on MVL ATRP and silver nanoparticles;Microchemical Journal;2023-08

2. Phosphatidylcholine-Specific Phospholipase C as a Promising Drug Target;Molecules;2023-07-25

3. An ultrasensitive electrochemical sensor for phospholipase C via signal amplification based on breathing ATRP and its application;The Analyst;2023

4. A new update of MALDI-TOF mass spectrometry in lipid research;Progress in Lipid Research;2022-04