1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay-Reference-Cited by-同舟云学术

1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay

Published:2016 Issue:4 Volume:141 Page:1331-1338
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Aparin Ilya O.¹²³,Farzan Valentina M.⁴⁵³,Veselova Olga A.⁴⁵³,Chistov Alexey A.¹²³,Podkolzin Alexander T.⁴⁵³,Ustinov Alexey V.¹²³,Shipulin German A.⁴⁵³,Formanovsky Andrey A.¹²³,Korshun Vladimir A.¹²³⁶⁷,Zatsepin Timofei S.⁴⁵³⁸⁹

Affiliation:

1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

2. 117997 Moscow

3. Russia

4. Central Research Institute of Epidemiology

5. 111123 Moscow

6. Gause Institute of New Antibiotics

7. 119021 Moscow

8. Department of Chemistry

9. Lomonosov Moscow State University

Abstract

We demonstrated that 1-phenylethynylpyrene (PEPy) is a superior substitute for AMCA and Alexa-350 as a short wavelength fluorescent dye for end-point PCR and quantitative PCR. This study broadens the panel of fluorescent dyes suitable for the use in Taqman probes.

Funder

Russian Foundation for Basic Research

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/AN/C5AN01767J

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