Stronger together for in-cell translation: natural and unnatural base modified mRNA-Reference-Cited by-同舟云学术

Stronger together for in-cell translation: natural and unnatural base modified mRNA

Published:2022 Issue:17 Volume:13 Page:4753-4761
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Bornewasser Lisa¹^ORCID,Domnick Christof¹,Kath-Schorr Stephanie¹^ORCID

Affiliation:

1. Institute of Organic Chemistry, Department of Chemistry, University of Cologne, Greinstrasse 4, 50939 Cologne, Germany

Abstract

Introducing unnatural base modifications site-specifically into the 3′-UTR of an mRNA bearing natural base modifications allows efficient visualization in cells by click chemistry. An enhanced protein expression in cells is observed from this twofold modified mRNA.

Funder

Boehringer Ingelheim Stiftung

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/SC/D2SC00670G

Reference58 articles.

1. Brief review of the mRNA vaccines COVID-19

2. Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability