Functionalization of gold nanoparticles with nanobodies through physical adsorption-Reference-Cited by-同舟云学术

Functionalization of gold nanoparticles with nanobodies through physical adsorption

Published:2017 Issue:23 Volume:9 Page:3430-3440
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Goossens Julie¹²³⁴⁵^ORCID,Sein Hla¹²³⁴⁵,Lu Shaohong⁶⁷⁸⁹,Radwanska Magdalena¹⁰¹¹¹²,Muyldermans Serge¹²³⁴⁵,Sterckx Yann G.-J.¹²³⁴⁵,Magez Stefan¹²³⁴⁵

Affiliation:

1. Research Unit for Cellular and Molecular Immunology (CMIM)

2. VUB

3. B-1050 Brussels

4. Belgium

5. Structural Biology Research Center (SBRC)

6. Institute of Parasitic Diseases

7. Zhejiang Academy of Medical Sciences

8. Hangzhou 310013

9. China

10. Ghent University Global Campus

11. 406-840 Incheon

12. South Korea

Abstract

The physical adsorption behaviour of nanobodies to gold nanoparticles is described for the first time in an extensive manner including parameters such as pI, ionic strength and several protein engineering strategies. The generated nanobody-gold conjugates are functional.

Funder

Vrije Universiteit Brussel

Agentschap voor Innovatie door Wetenschap en Technologie

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2017/AY/C7AY00854F

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1. Laboratory medicine: an essential partner in the care of cancer patients

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