Naked-eye sensitive detection of alkaline phosphatase (ALP) and pyrophosphate (PPi) based on a horseradish peroxidase catalytic colorimetric system with Cu(ii)-Reference-Cited by-同舟云学术

Naked-eye sensitive detection of alkaline phosphatase (ALP) and pyrophosphate (PPi) based on a horseradish peroxidase catalytic colorimetric system with Cu(ii)

Published:2016 Issue:19 Volume:141 Page:5549-5554
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Shi Dongmin¹²³⁴⁵,Sun Yue¹²³⁴⁵,Lin Lin¹²³⁴⁵,Shi Chunjun¹²³⁴⁵,Wang Guangfeng¹²³⁴⁵,Zhang Xiaojun¹²³⁴⁵

Affiliation:

1. Key Laboratory of Chem-Biosensing

2. Anhui province; Key Laboratory of Functional Molecular Solids

3. Anhui province; College of Chemistry and Materials Science

4. Center for Nano Science and Technology

5. Anhui Normal University

Abstract

In this paper, a novel colorimetric method for the detection of alkaline phosphatase (ALP) and pyrophosphate (PPi) was designed based on a Cu²⁺–horseradish peroxidase (HRP)–3,3′,5,5′-tetra-methylbenzidine (TMB)–H₂O₂ system.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/AN/C6AN01124A

Reference33 articles.

1. Structure and Mechanism of Alkaline Phosphatase

2. Clinical and biological significance of an isozyme tumor marker—PLAP

3. Alkaline Phosphatase

4. High-molecular intestinal alkaline phosphatase in chronic liver diseases

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