Multiplex real-time PCR using double-strand primers and probes for the detection of nucleic acids-Reference-Cited by-同舟云学术

Multiplex real-time PCR using double-strand primers and probes for the detection of nucleic acids

Published:2020 Issue:44 Volume:12 Page:5392-5396
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Zhang Zhang¹²³⁴⁵^ORCID,Yao Juan³⁶⁷⁸^ORCID,Huang Xiaoling³⁶⁷⁸,Zhang Li¹²³⁴⁵^ORCID,Wang Ting¹²³⁴⁵,Weng Zhi¹²³⁴⁵,Xie Guoming¹²³⁴⁵

Affiliation:

1. Key Laboratory of Laboratory Medical Diagnostics

2. Ministry of Education

3. Department of Laboratory Medicine

4. Chongqing Medical University

5. Chongqing

6. The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University

7. Luzhou

8. China

Abstract

Double strand primer-mediated strand displacement reaction was used for the construction of multiplex RT-PCR; double strand primers act as a function of both the primer and probe.

Funder

National Natural Science Foundation of China

Southern Medical University

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/AY/D0AY01661F

Reference31 articles.

1. Multiplex PCR: advantages, development, and applications.

2. Multiplex PCR: Optimization and Application in Diagnostic Virology

3. Combining multiplex PCR and high-resolution melting for the detection and discrimination of arthropod transmitted viruses of cereals

4. MPprimer: a program for reliable multiplex PCR primer design

5. Efficient primer design algorithms

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