Limited-resource preparable chitosan magnetic particles for extracting amplification-ready nucleic acid from complex biofluids

Author:

Tripathy Sayantan1,Chalana Ashish Kumar1,Talukdar Arunansu2,Rajesh P. V.3,Saha Abhijit3ORCID,Pramanik Goutam3,Ghosh Souradyuti14ORCID

Affiliation:

1. Department of Chemistry, Bennett University, Greater Noida, Uttar Pradesh 201310, India

2. Department of Medicine, Medical College and Hospital, Kolkata, West Bengal, India

3. UGC-DAE CSR, Kolkata Centre, Sector III, LB-8, Bidhan Nagar, Kolkata – 700 106, India

4. Department of Biotechnology, Bennett University, Greater Noida, Uttar Pradesh 201310, India

Abstract

We present methods for limited-resource-friendly preparation of chitosan magnetic particles that are amenable to ultrasensitive downstream isothermal and conventional nucleic acid amplification tests.

Funder

Mission on Nano Science and Technology

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Reference46 articles.

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3. Institute of Medicine (US) Forum on Microbial Threats , S.Knobler , A.Mahmoud , S.Lemon and L.Pray , The Impact of Globalization on Infectious Disease Emergence and Control: Exploring the Consequences and Opportunities: Workshop Summary , National Academies Press , USA , 2006

4. J. A.Patz and M. B.Hahn , in One Health: The Human-Animal-Environment Interfaces in Emerging Infectious Diseases: Food Safety and Security, and International and National Plans for Implementation of One Health Activities , ed. J. S. Mackenzie , M. Jeggo , P. Daszak and J. A. Richt , Springer , Berlin, Heidelberg , 2013 , pp. 141–171

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