Rapid detection of HPV16/18 based on a CRISPR-Cas13a/Cas12a dual-channel system-Reference-Cited by-同舟云学术

Rapid detection of HPV16/18 based on a CRISPR-Cas13a/Cas12a dual-channel system

Published:2022 Issue:48 Volume:14 Page:5065-5075
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Zheng Xue¹,Li Yuankun²,Yuan Mingzhu¹,Shen Yue¹,Chen Shuaiyin¹^ORCID,Duan Guangcai¹

Affiliation:

1. College of Public Health, Zhengzhou University, Zhengzhou, 450000, China

2. Obstetrics and Gynecology Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, 450014, China

Abstract

Orthogonal cleavage of DNA and RNA reporters by target-activated Cas12a/Cas13a induces two-colored fluorescence emission (JOE-FAM), which can be detected using a standard fluorometer or observed by a dual-channel visualization detection platform.

Funder

National Natural Science Foundation of China

Henan Provincial Science and Technology Research Project

National Science and Technology Planning Project

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AY/D2AY01536F

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