A continuous flow PCR array microfluidic chip applied for simultaneous amplification of target genes of periodontal pathogens-Reference-Cited by-同舟云学术

A continuous flow PCR array microfluidic chip applied for simultaneous amplification of target genes of periodontal pathogens

Published:2022 Issue: Volume: Page:
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Yang Bo¹,Wang Ping²,Li Zhenqing¹^ORCID,Tao Chunxian¹,You Qingxiang¹,Sekine Shinichi³^ORCID,Zhuang Songlin¹,Zhang Dawei¹^ORCID,Yamaguchi Yoshinori⁴

Affiliation:

1. Engineering Research Center of Optical Instrument and System, Key Lab of Optical Instruments and Equipment for Medical Engineering, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China

2. Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, China

3. Department of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Osaka 565-0871, Japan

4. Oono Joint Research laboratory, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan

Abstract

The concept of time to place conversion makes using a continuous flow polymerase chain reaction microfluidic chip an ideal way to reduce the time required for amplification of target genes; however, it also brings about low throughput amplicons.

Funder

Science and Technology Commission of Shanghai Municipality

University of Shanghai for Science and Technology

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D1LC00814E

Reference23 articles.

1. Oral Diseases affect some 3.9 Billion people

2. Association between periodontal pathogens and systemic disease