Dispersion-enhancing surface treatment of AuNPs for a reduced probe loading and detection limit using t-SPR detection

Author:

Yeung Wing Kiu1,Lo Shu-Cheng12,Wang Sheng-Hann1,Wei Pei-Kuen1,Cheng Ji-Yen1345ORCID

Affiliation:

1. Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan

2. Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan

3. Institute of Biophotonics, National Yang Ming Chiao Tung University, Taipei, Taiwan

4. Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Taiwan

5. College of Engineering, Chang Gung University, Taoyuan, Taiwan

Abstract

A nanoplasmonic platform for oligonucleotide detection is developed. Functionalized unmodified PEG-Au was used as an SPR signal amplifier. The SPR sensor exhibited high sensitivity with a detection limit of 100 aM. The platform was able to detect a single mismatch sequence.

Funder

Ministry of Science and Technology, Taiwan

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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