Cytosolic delivery of CRISPR/Cas9 ribonucleoproteins for genome editing using chitosan-coated red fluorescent protein-Reference-Cited by-同舟云学术

Cytosolic delivery of CRISPR/Cas9 ribonucleoproteins for genome editing using chitosan-coated red fluorescent protein

Published:2019 Issue:32 Volume:55 Page:4707-4710
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Qiao Jie¹²³⁴⁵^ORCID,Sun Wenli¹²³⁴,Lin Siyu¹²³⁴,Jin Rong⁶⁷⁸⁴,Ma Lixin¹²³⁴⁵,Liu Yi¹²³⁴⁵^ORCID

Affiliation:

1. State Key Laboratory of Biocatalysis and Enzyme Engineering

2. School of Life Sciences

3. Hubei University

4. China

5. Hubei Key Laboratory of Industrial Biotechnology

6. Institute of Nanochemistry & Nanobiology

7. Shanghai University

8. Shanghai

Abstract

We report chitosan-coated red fluorescent protein nanoparticles that can simultaneously deliver Cas9 RNPs and DNA donors to the cells for efficient genome editing via the HDR or NHEJ pathway with high efficacy and non-cytotoxicity.

Funder

Natural Science Foundation of Hubei Province

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2019/CC/C9CC00010K

Reference36 articles.

1. The new frontier of genome engineering with CRISPR-Cas9

2. Development and Applications of CRISPR-Cas9 for Genome Engineering

3. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

4. Therapeutic genome editing: prospects and challenges

5. Implications of human genetic variation in CRISPR-based therapeutic genome editing

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