In situ assessment of mitochondrial respiratory activity and lipid metabolism of mouse oocytes using resonance Raman spectroscopy

Author:

Ishigaki Mika12ORCID,Kashiwagi Shinsuke3,Wakabayashi Satoru3,Hoshino Yumi4

Affiliation:

1. Institute of Agricultural and Life Sciences, Academic Assembly, Shimane University, 1060 Nishikawatsu, Matsue, Shimane, 690-8504, Japan

2. Raman Project Center for Medical and Biological Applications, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan

3. Bio/Life Science Project, Sales Division, HORIBA, Ltd, 2 Miyanohigashi-cho, Kisshoin, Minami-ku, Kyoto 601-8510, Japan

4. Laboratory of Animal Reproduction, Graduate School of Integrated Science for Life, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan

Abstract

The respiratory activity and lipid metabolism depending on the degree of oocyte maturation can be assessed using resonance Raman spectroscopy in a non-invasive manner.

Funder

Japan Society for the Promotion of Science

Adaptable and Seamless Technology Transfer Program through Target-Driven R and D

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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