Quantification of biomolecules responsible for biomarkers in the surface-enhanced Raman spectra of bacteria using liquid chromatography-mass spectrometry

Author:

Chiu Shirley Wen-Yu12345ORCID,Cheng Ho-Wen123,Chen Zhi-Xin123,Wang Huai-Hsien123,Lai Ming-Yu123,Wang Juen-Kai12362,Wang Yuh-Lin12372ORCID

Affiliation:

1. Institute of Atomic and Molecular Sciences, Academia Sinica

2. Taipei 10617

3. Taiwan

4. Department of Chemistry, National Tsing Hua University

5. Hsinchu 30013

6. Center for Condensed Matter Sciences, National Taiwan University

7. Department of Physics, National Taiwan University

Abstract

Biomarkers in SERS spectra of bacteria originate from bacterial purine metabolites, whose quantification indicates their continuous release in a stressful water environment.

Funder

Ministry of Science and Technology, Taiwan

Academia Sinica

Publisher

Royal Society of Chemistry (RSC)

Subject

Physical and Theoretical Chemistry,General Physics and Astronomy

Reference36 articles.

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4. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA

5. Mass spectrometry tools for the classification and identification of bacteria

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