Comparative analysis of culture- and ddPCR-based wastewater surveillance for carbapenem-resistant bacteria-Reference-Cited by-同舟云学术

Comparative analysis of culture- and ddPCR-based wastewater surveillance for carbapenem-resistant bacteria

Published:2024 Issue: Volume: Page:
ISSN:2053-1400
Container-title:Environmental Science: Water Research & Technology
language:en
Short-container-title:Environ. Sci.: Water Res. Technol.

Author:

Zhou Siyi¹^ORCID,Lou Esther G.¹^ORCID,Schedler Julia²^ORCID,Ensor Katherine B.²,Hopkins Loren²³,Stadler Lauren B.¹^ORCID

Affiliation:

1. Department of Civil and Environmental Engineering, Rice University, 6100 Main Street MS 352, Houston, TX 77005, USA

2. Department of Statistics, Rice University, 6100 Main Street MS 138, Houston, TX 77005, USA

3. Houston Health Department, 8000 N. Stadium Dr., Houston, TX 77054, USA

Abstract

This study compares culture-based and ddPCR methods for quantifying carbapenem resistance in wastewater, revealing weak correlations. Nanopore sequencing clarifies resistance mechanisms, emphasizing method selection and integrated AR surveillance.

Funder

Centers for Disease Control and Prevention Foundation

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/EW/D4EW00525B

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