A generic approach based on long-lifetime fluorophores for the assessment of protein binding to polymer nanoparticles by fluorescence anisotropy-Reference-Cited by-同舟云学术

A generic approach based on long-lifetime fluorophores for the assessment of protein binding to polymer nanoparticles by fluorescence anisotropy

Published:2024 Issue:7 Volume:16 Page:3659-3667
ISSN:2040-3364
Container-title:Nanoscale
language:en
Short-container-title:Nanoscale

Author:

Ahmed Marwa A.¹²,Hessz Dóra³⁴,Gyarmati Benjámin³^ORCID,Páncsics Mirkó¹,Kovács Norbert¹^ORCID,Gyurcsányi Róbert E.¹⁵⁶,Kubinyi Miklós³^ORCID,Horváth Viola¹⁶^ORCID

Affiliation:

1. Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3., H-1111 Budapest, Hungary

2. Department of Chemistry, Faculty of Science, Arish University, 45511 El-Arish, North Sinai, Dahyet El Salam, Egypt

3. Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3., H-1111 Budapest, Hungary

4. MTA-BME “Lendület” Quantum Chemistry Research Group, Műegyetem rkp. 3., H-1111 Budapest, Hungary

5. MTA-BME “Lendület” Chemical Nanosensors Research Group, Műegyetem rkp. 3., H-1111 Budapest, Hungary

6. ELKH-BME Computation Driven Chemistry Research Group, Műegyetem rkp. 3., H-1111 Budapest, Hungary

Abstract

Using long-lifetime fluorophores as protein labels, protein–nanoparticle interactions can be monitored through anisotropy change. Besides gaining thermodynamic and kinetic information on the binding process, competitive protein assays can be set up.

Funder

Magyar Tudományos Akadémia

National Research, Development and Innovation Office

Nemzeti Kutatási, Fejlesztési és Innovaciós Alap

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/NR/D3NR02460A

Reference42 articles.

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