Characterization of ovarian cancer-derived extracellular vesicles by surface-enhanced Raman spectroscopy

Author:

Ćulum Nina M.1,Cooper Tyler T.2ORCID,Lajoie Gilles A.2,Dayarathna Thamara3,Pasternak Stephen H.3ORCID,Liu Jiahui4,Fu Yangxin4,Postovit Lynne-Marie45,Lagugné-Labarthet François1ORCID

Affiliation:

1. University of Western Ontario (Western University), Department of Chemistry, 1151 Richmond St., London, Ontario, N6A 5B7, Canada

2. University of Western Ontario (Western University), Department of Biochemistry, 1151 Richmond St., London, Ontario, N6A 5B7, Canada

3. University of Western Ontario (Western University), Robarts Research Institute, 1151 Richmond St., London, Ontario, N6A 5B7, Canada

4. University of Alberta, Department of Oncology, 116 St. & 85 Ave., Edmonton, Alberta, T6G 2R3, Canada

5. Queen's University, Department of Biomedical & Molecular Sciences, 99 University Ave., Kingston, Ontario, K2L 3N6, Canada

Abstract

EVs derived from normal ovarian (hIOSE) and ovarian cancer (OVCAR3, OV-90, EOC6, EOC18) cells were analysed by SERS. The SERS spectra of normal and cancer EVs were differentiated by PCA and machine learning, through which we achieved >99% accuracy.

Funder

Natural Sciences and Engineering Research Council of Canada

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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