Direct immunoassay on a polyester microwell plate for colorimetric detection of the spike protein in swab and saliva samples

Author:

Moreira Nikaele S.1,Baldo Thaisa A.1,Duarte Lucas C.12ORCID,Lopes-Luz Leonardo34,Oliveira Karoliny A.1,Estrela Paulo F. N.1ORCID,Simões Amanda M.1,Bührer-Sékula Samira34ORCID,Duarte Gabriela R. M.14ORCID,Coltro Wendell K. T.145ORCID

Affiliation:

1. Instituto de Química, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil

2. Instituto Federal de Educação, Ciência e Tecnologia de Goiás – Campus Inhumas, 75402-556, Inhumas, GO, Brazil

3. Instituto de Patologia Tropical e Saúde Pública, Centro Multiusuário de Bioinsumos e Tecnologias em Saúde, Universidade Federal de Goiás, 74605-050, Goiânia, GO, Brazil

4. Innovation Hub in Point-of-Care Technologies, 74690-900, Goiânia, GO, Brazil

5. Instituto Nacional de Ciência e Tecnologia de Bioanalítica, 13084-971, Campinas, SP, Brazil

Abstract

This study presents the development of a polyester microplate for detecting the S-protein of the SARS-CoV-2 virus in saliva and nasopharyngeal swab samples using direct enzyme-linked immunosorbent assay (ELISA) technology.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Instituto Nacional de Ciência e Tecnologia de Bioanalítica

Fundação de Amparo à Pesquisa do Estado de Goiás

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Reference40 articles.

1. Coronavirus disease (COVID-19) , https://www.who.int/emergencies/diseases/novel-coronavirus-2019 , (accessed 21 September 2023)

2. SARS-CoV-2 diagnostic testing rates determine the sensitivity of genomic surveillance programs

3. Detection of COVID-19: A review of the current literature and future perspectives

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