Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles

Author:

Sklenárová Dorota1,Hlaváček Antonín2,Křivánková Jana2,Brandmeier Julian C.13ORCID,Weisová Julie2,Řiháček Michal4,Gorris Hans H.1ORCID,Skládal Petr1,Farka Zdeněk1ORCID

Affiliation:

1. Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic

2. Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 97, 602 00 Brno, Czech Republic

3. Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 930 53 Regensburg, Germany

4. Department of Laboratory Methods, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic

Abstract

A microfluidic device for single-molecule detection of prostate-specific antigen was developed, utilizing magnetic microparticles as a solid phase and photon-upconversion nanoparticles as a label.

Funder

Ústav analytické chemie, Akademie Věd České Republiky

Grantová Agentura České Republiky

Publisher

Royal Society of Chemistry (RSC)

Reference49 articles.

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5. S.Hosseini , P.Vázquez-Villegas , M.Rito-Palomares and S. O.Martinez-Chapa , in Enzyme-linked Immunosorbent Assay (ELISA): From A to Z , ed. S. Hosseini , P. Vázquez-Villegas , M. Rito-Palomares and S. O. Martinez-Chapa , Springer , Singapore , 2018 , pp. 67–115

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