Sub-7-second genotyping of single-nucleotide polymorphism by high-resolution melting curve analysis on a thermal digital microfluidic device-Reference-Cited by-同舟云学术

Sub-7-second genotyping of single-nucleotide polymorphism by high-resolution melting curve analysis on a thermal digital microfluidic device

Published:2016 Issue:4 Volume:16 Page:743-752
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Chen Tianlan¹²³⁴⁵,Jia Yanwei¹²³⁴,Dong Cheng¹²³⁴⁵,Gao Jie¹²³⁴⁵,Mak Pui-In¹²³⁴⁵,Martins Rui P.¹²³⁴⁵

Affiliation:

1. State-Key Laboratory of Analog and Mixed-Signal VLSI

2. University of Macau

3. Macao

4. China

5. Faculty of Science and Technology – ECE

Abstract

A novel thermal digital microfluidic (T-DMF) device enables precise thermal modulation and pipelined measurement of multiple samples. Ultrafast DNA melting curve analysis is achieved in less than 7 seconds, with the resolution adequate for single-nucleotide discrimination.

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2016/LC/C5LC01533B

Reference51 articles.

1. Advances in microfluidic PCR for point-of-care infectious disease diagnostics

2. Nucleic acid amplification using microfluidic systems

3. Portable nucleic acid thermocyclers

4. Micro-chemical synthesis of molecular probes on an electronic microfluidic device

5. Droplet-Scale Estrogen Assays in Breast Tissue, Blood, and Serum

Cited by 23 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A cost-effective and field-deployable sensing system for chip-integrated detection of bacteria with the naked eye;Sensors and Actuators B: Chemical;2024-07

2. DNA melting analysis;Molecular Aspects of Medicine;2024-06

3. Drug screening on digital microfluidics for cancer precision medicine;Nature Communications;2024-05-22

4. Sub-5-Minute Ultrafast PCR using Digital Microfluidics;Biosensors and Bioelectronics;2023-12

5. Continuous gradient temperature control of microfluidic chip based on thermoelectric cooler;Applied Thermal Engineering;2023-11