De novo sequencing and native mass spectrometry revealed hetero-association of dirigent protein homologs and potential interacting proteins in Forsythia × intermedia-Reference-Cited by-同舟云学术

De novo sequencing and native mass spectrometry revealed hetero-association of dirigent protein homologs and potential interacting proteins in Forsythia × intermedia

Published:2021 Issue:24 Volume:146 Page:7670-7681
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zhou Mowei¹^ORCID,Laureanti Joseph A.²,Bell Callum J.³^ORCID,Kwon Mi⁴,Meng Qingyan⁴^ORCID,Novikova Irina V.¹,Thomas Dennis G.⁵,Nicora Carrie D.⁵^ORCID,Sontag Ryan L.⁵,Bedgar Diana L.⁴,O'Bryon Isabelle⁶,Merkley Eric D.⁶^ORCID,Ginovska Bojana²,Cort John R.⁴⁵^ORCID,Davin Laurence B.⁴^ORCID,Lewis Norman G.⁴^ORCID

Affiliation:

1. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA

2. Physical and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA

3. National Center for Genome Resources, Santa Fe, NM, USA

4. Institute of Biological Chemistry, Washington State University, Pullman, WA, USA

5. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA

6. Chemical and Biological Signatures Group, Pacific Northwest National Laboratory, Richland, WA, USA

Abstract

Dirigent proteins (DPs) were first discovered from Forsythia stems, but all of the co-purified proteins were unknown. De novo sequencing and native mass spectrometry identified additional proteins and heterocomplexes between two DP homologs.

Funder

Biological and Environmental Research

National Institute of Food and Agriculture

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN01476E

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4. Dirigent Protein Mode of Action Revealed by the Crystal Structure of AtDIR6

5. Opposite Stereoselectivities of Dirigent Proteins in Arabidopsis and Schizandra Species