Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting-Reference-Cited by-同舟云学术

Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting

Published:2022 Issue:33 Volume:13 Page:9739-9748
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Dai Yancen¹^ORCID,Sato Yuko²^ORCID,Zhu Bo³^ORCID,Kitaguchi Tetsuya³^ORCID,Kimura Hiroshi²^ORCID,Ghadessy Farid J.⁴^ORCID,Ueda Hiroshi³^ORCID

Affiliation:

1. Graduate School of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta-cho, Yokohama, Kanagawa 226-8503, Japan

2. Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology, Nagatsuta-cho, Yokohama, Kanagawa 226-8503, Japan

3. Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Nagatsuta-cho, Yokohama, Kanagawa 226-8503, Japan

4. Disease Intervention Technology Laboratory, Institute of Molecular and Cellular Biology, A*STAR, Singapore

Abstract

A fluorescent immunosensor that lights up tumor biomarker p53 in living cells was developed based on the Q-body technology. The technology was further applied to the live cell monitoring of p53 levels, and live cell sorting based on p53 expression.

Funder

Japan Science and Technology Agency

Japan Society for the Promotion of Science

Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering

Strategic International Collaborative Research Program

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/SC/D2SC02355E

Reference51 articles.