Formation of an instantaneous nick for highly efficient adenylation of oligonucleotides by ligase without subsequent jointing-Reference-Cited by-同舟云学术

Formation of an instantaneous nick for highly efficient adenylation of oligonucleotides by ligase without subsequent jointing

Published:2024 Issue:21 Volume:60 Page:2942-2945
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Hu Kunling¹,Sun Wenhua¹,Chen Hui¹,Luo Jian¹,Song Ziting¹,An Ran¹²^ORCID,Komiyama Makoto³,Liang Xingguo¹²^ORCID

Affiliation:

1. State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, No. 1299 Sansha Road, Qingdao, P. R. China

2. Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), No. 1 Wenhai Road, Qingdao, P. R. China

3. Research Center for Advanced Science and Technology (RCAST), The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan

Abstract

By forming a nick instantaneously, nucleic acids are efficiently adenylated by T4 DNA ligase without further ligation.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CC/D4CC00590B

Reference25 articles.

1. Enzymatic Breakage and Joining of Deoxyribonucleic Acid

2. Joining of ribooligonucleotides with T4 RNA ligase and identification of the oligonucleotide-adenylate intermediate.

3. Crystal Structure of an ATP-Dependent DNA Ligase from Bacteriophage T7

4. Efficient microRNA capture and bar-coding via enzymatic oligonucleotide adenylation

5. Efficient synthesis of stably adenylated DNA and RNA adapters for microRNA capture using T4 RNA ligase 1

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