Multispectral Bayesian reconstruction technique for real-time two color fluorescence microscopy

Author:

Dilipkumar Shilpa12345,Manjithaya Ravi6785,Mondal Partha Pratim12345

Affiliation:

1. Nanobioimaging Laboratory

2. Department of Instrumentation and Applied Physics

3. Indian Institute of Science

4. Bangalore 560012

5. India

6. Molecular Biology and Genetics Unit

7. Jawaharlal Nehru Center for Advanced Scientific Research

8. Bangalore 560064

Abstract

We have developed a real-time imaging method for two-color widefield fluorescence microscopy using a combined approach that integrates multi-spectral imaging and Bayesian image reconstruction technique.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

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