High performance anion exchange chromatographic and colorimetric methods for quality assessment of total and free polysaccharide content in <i>Haemophilus influenzae</i> type b conjugate vaccine containing lactose-Reference-Cited by-同舟云学术

登录注册会员服务联系我们

High performance anion exchange chromatographic and colorimetric methods for quality assessment of total and free polysaccharide content in Haemophilus influenzae type b conjugate vaccine containing lactose

Published:2022 Issue:38 Volume:14 Page:3757-3765
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Hussein Asmaa R.¹^ORCID,Rezk Mamdouh R.²,Fathalla Faten Abdel Aziz¹,El-Saharty Yasser S.²,Nadim Ahmed H.²

Affiliation:

1. National Organization for Research and Control of Biologicals, Egypt

2. Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt

Abstract

Ultrafiltration or dialysis was used as sample pretreatment to remove 95% of lactose. Free PRP was separated using SPE, and then total and free PRP were analyzed using HPAEC-PAD (ribitol determination) and a colorimetric assay (phosphorus determination).

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AY/D2AY00937D

Reference27 articles.

1. R. W.Ellis and D. M.Granoff , Development and Clinical Uses of Haemophilus b Conjugate Vaccines , Informa Health Care , 1994 , vol. 11

2. Decline of Childhood Haemophilus influenzae Type b (Hib) Disease in the Hib Vaccine Era

3. Epidemiology of Haemophilus influenzae Type b Infections

4. In European Pharmacopoeia , 2020

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线，采集、加工和组织学术论文而形成的新型学术文献查询和分析系统，可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容，当前同舟云学术共收录了国内外主流学术期刊6万余种，收集的期刊论文及会议论文总量共计约1.5亿篇，并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询！咨询电话：010-8811{复制后删除}0370

www.globalauthorid.com

TOP