Amplitude multiplexed wastewater surveillance for campus health: tracking SARS-CoV-2, influenza A, and norovirus

Author:

Saldana Michael A.1ORCID,Geng Jiayu1,Shen Litao1,Ghanem-Uzqueda Angie2ORCID,Van Orman Sarah2,Tilley Kimberly B.2,Sun Dick3,Willes Deona3,Smith Adam L.1ORCID

Affiliation:

1. Sonny Astani Department of Civil and Environmental Engineering, University of Southern California, 920 Downey Way, BHE 201, Los Angeles, CA 90089, USA

2. Keck School of Medicine, University of Southern California, 1975 Zonal Ave, Los Angeles, CA 90033, USA

3. Environmental Health and Safety, University of Southern California, 3434 S. Grand Avenue, CAL 120, Los Angeles, CA 90089, USA

Abstract

This study implements wastewater surveillance on a university campus to monitor highly infectious communal diseases utilizing ddPCR and overcoming the limitation of two fluorescent channels of a ddPCR reader by employing an amplitude multiplex.

Funder

University of Southern California

Publisher

Royal Society of Chemistry (RSC)

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