An amplification-free, 16S rRNA test for <i>Neisseria gonorrhoeae</i> in urine-Reference-Cited by-同舟云学术

An amplification-free, 16S rRNA test for Neisseria gonorrhoeae in urine

Published:2023 Issue:1 Volume:2 Page:163-167
ISSN:2635-0998
Container-title:Sensors & Diagnostics
language:en
Short-container-title:Sens. Diagn.

Author:

Zheng Zhenrong¹,Cao Yan¹,Chandrasekaran Sukantha²,Schmidt Jacob J.³,Garner Omai B.²,Monbouquette Harold G.¹^ORCID

Affiliation:

1. Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA

2. Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA

3. Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA

Abstract

Conductometric detection of N. gonorrhoeae 16S rRNA (green) by blockade of glass pores. Polystyrene beads conjugated with PNA probe (orange) are charge neutral but acquire negative charge and electrophoretic mobility upon hybridization of rRNA.

Funder

National Institute of Allergy and Infectious Diseases

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2023/SD/D2SD00128D

Reference13 articles.

1. CDC, Sexually Transmitted Disease Surveillance 2020, https://www.cdc.gov/std/statistics/2020/overview.htm#Gonorrhea , (accessed July 19, 2022 )

2. Rising to Meet the Programmatic Public Health Challenges of Emerging Neisseria gonorrhoeae Antimicrobial Resistance: Strengthening the United States Response to Resistant Gonorrhea

3. CDC, Gonorrhea—CDC Detailed Fact Sheet, https://www.cdc.gov/std/gonorrhea/stdfact-gonorrhea-detailed.htm , (accessed July 19, 2022 )

4. The Laboratory Diagnosis ofNeisseria gonorrhoeae

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1. Correction: An amplification-free, 16S rRNA test for Neisseria gonorrhoeae in urine;Sensors & Diagnostics;2023