Comparison of multiple protein extraction buffers for GeLC-MS/MS proteomic analysis of liver and colon formalin-fixed, paraffin-embedded tissues-Reference-Cited by-同舟云学术

Comparison of multiple protein extraction buffers for GeLC-MS/MS proteomic analysis of liver and colon formalin-fixed, paraffin-embedded tissues

Published:2016 Issue:2 Volume:12 Page:553-565
ISSN:1742-206X
Container-title:Molecular BioSystems
language:en
Short-container-title:Mol. BioSyst.

Author:

Broeckx Valérie¹²³⁴⁵,Boonen Kurt¹²³⁴⁵,Pringels Lentel¹²³⁴⁵,Sagaert Xavier⁶⁷⁵⁸,Prenen Hans⁹¹⁰⁷⁵⁸,Landuyt Bart¹²³⁴⁵,Schoofs Liliane¹²³⁴⁵,Maes Evelyne¹¹¹²⁸¹³¹⁴

Affiliation:

1. Research Group of Functional Genomics and Proteomics

2. Department of Biology

3. University of Leuven

4. Zoological Institute

5. 3000 Leuven

6. Centre for Translational Cell and Tissue Research

7. University Hospital of Leuven

8. Belgium

9. Department of Gastro-Enterology

10. Digestive Oncology Unit

11. Flemish Institute for Technological Research (VITO)

12. 2400 Mol

13. Centre for Proteomics

14. University of Antwerp/Flemish Institute for Technological Research (VITO)

Abstract

Comparison of protein extraction buffers and overall variation in formalin-fixed paraffin-embedded tissue using the same proteomic workflow.

Funder

Onderzoeksraad, KU Leuven

Publisher

Royal Society of Chemistry (RSC)

Subject

Molecular Biology,Biotechnology

Link

http://pubs.rsc.org/en/content/articlepdf/2016/MB/C5MB00670H

Reference55 articles.

1. Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges

2. Proteomics on Fixed Tissue Specimens – A Review

3. Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues

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