Unveiling the intricacy of gapmer oligonucleotides through advanced tandem mass spectrometry approaches and scan accumulation for 2DMS-Reference-Cited by-同舟云学术

Unveiling the intricacy of gapmer oligonucleotides through advanced tandem mass spectrometry approaches and scan accumulation for 2DMS

Published:2024 Issue:18 Volume:149 Page:4687-4701
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Rahman Mohammed¹²,Marzullo Bryan P.¹,Lam Pui Yiu¹^ORCID,Barrow Mark P.¹^ORCID,Holman Stephen W.³,Ray Andrew D.⁴^ORCID,O'Connor Peter B.¹^ORCID

Affiliation:

1. Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK

2. Department of Physics, University of Warwick, Coventry, CV4 7AL, UK

3. Chemical Development, Pharmaceutical Technology & Development, Operations, AstraZeneca, AstraZeneca, SK10 2NA, UK

4. New Modalities & Parental Development, Pharmaceutical Technology & Development, Operations, AstraZeneca, Macclesfield, SK10 2NA, UK

Abstract

Multimodal tandem mass spectrometry sequencing of oligonucleotides using CID, EDD, IRMPD, and UVPD as fragmentation methods, and using 2-dimensional mass spectrometry with signal averaging to differentiate fragments from closely spaced precursors.

Funder

University of Warwick

Engineering and Physical Sciences Research Council

Biotechnology and Biological Sciences Research Council

Horizon 2020 Framework Programme

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AN/D4AN00484A

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