An electrochemical biosensor for the activity assay of polynucleotide kinase and inhibitor screening-Reference-Cited by-同舟云学术

An electrochemical biosensor for the activity assay of polynucleotide kinase and inhibitor screening

Published:2015 Issue:23 Volume:7 Page:9984-9991
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Zhang Qingming¹²³⁴,Li Xue⁵⁶⁷⁴,Li Bingchen⁵⁶⁷⁴,Yin Huanshun⁵⁶⁷⁴,Ai Shiyun⁵⁶⁷⁴

Affiliation:

1. College of Chemistry and Pharmaceutical Science

2. Qingdao Agricultural University

3. Qingdao 266109

4. PR China

5. College of Chemistry and Material Science

6. Shandong Agricultural University

7. Taian

Abstract

A sensitive and selective electrochemical biosensor was fabricated for polynucleotide kinase (PNK) activity assay and inhibitor screening based on phos-tag-biotin mediated double signal amplification.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/AY/C5AY02295A

Reference26 articles.

1. Mechanisms of RecQ helicases in pathways of DNA metabolism and maintenance of genomic stability

2. Mutations in PNKP cause microcephaly, seizures and defects in DNA repair

3. Genetic Screening for Synthetic Lethal Partners of Polynucleotide Kinase/Phosphatase: Potential for Targeting SHP-1–Depleted Cancers

4. Identification of a Small Molecule Inhibitor of the Human DNA Repair Enzyme Polynucleotide Kinase/Phosphatase

5. Bacteriophage T4 anticodon nuclease, polynucleotide kinase and RNA ligase reprocess the host lysine tRNA.

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