IL-2 secretion-based sorting of single T cells using high-throughput microfluidic on-cell cytokine capture-Reference-Cited by-同舟云学术

IL-2 secretion-based sorting of single T cells using high-throughput microfluidic on-cell cytokine capture

Published:2022 Issue:8 Volume:22 Page:1576-1583
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Dimatteo Robert¹²,Di Carlo Dino²³⁴^ORCID

Affiliation:

1. Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, P.O. Box 951592, Los Angeles, CA, 90095, USA

2. Department of Bioengineering, University of California, Los Angeles, 420 Westwood Plaza, 5121 Engineering V, P.O. Box 951600, Los Angeles, CA 90095, USA

3. California Nano Systems Institute, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095, USA

4. Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095, USA

Abstract

Secretion encoding workflow in which cells are co-encapsulated with anti-cytokine capture and detection probes in drops using a parallelized step emulsification device. Cells recovered from the emulsion are analysed and sorted by flow cytometry.

Funder

National Cancer Institute

National Institutes of Health

Jonsson Comprehensive Cancer Center

David Geffen School of Medicine, University of California, Los Angeles

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D1LC01098K

Reference35 articles.