Rapid isolation of antigen-specific B-cells using droplet microfluidics-Reference-Cited by-同舟云学术

Rapid isolation of antigen-specific B-cells using droplet microfluidics

Published:2020 Issue:45 Volume:10 Page:27006-27013
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Ding Ruihua¹²³⁴⁵,Hung Kuo-Chan¹²³⁴^ORCID,Mitra Anindita¹²³⁴,Ung Lloyd W.¹²³⁴,Lightwood Daniel⁶⁷,Tu Ran¹²³⁴⁸,Starkie Dale⁶⁷,Cai Liheng¹²³⁴⁹^ORCID,Mazutis Linas¹²³⁴¹⁰^ORCID,Chong Shaorong¹¹⁴¹²³⁴,Weitz David A.¹²³⁴¹³^ORCID,Heyman John A.¹²³⁴¹⁴^ORCID

Affiliation:

1. John A. Paulson School of Engineering and Applied Sciences

2. Harvard University

3. Cambridge

4. USA

5. Department of Chemistry and Chemical Biology

6. UCB Pharma

7. UK

8. CAS Key Laboratory of Systems Microbial Biotechnology

9. Department of Materials Science and Engineering

10. Vilnius University

11. New England Biolabs

12. Arbor Biotechnologies

13. Department of Physics

14. SphereBio

Abstract

We use a droplet-microfluidics-based platform to rapidly identify and isolate individual primary cells that secrete desired antibodies. We then retrieve the antibody-encoding sequences and create recombinant antibodies that bind the target protein.

Funder

Massachusetts Life Sciences Center

Division of Cancer Prevention, National Cancer Institute

National Institutes of Health

Defense Advanced Research Projects Agency

European Social Fund

Bill and Melinda Gates Foundation

Solvay Pharmaceuticals

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry