Real-time investigation of human topoisomerase I reaction kinetics using an optical sensor: a fast method for drug screening and determination of active enzyme concentrations
Author:
Affiliation:
1. Department of Molecular Biology and Genetics
2. Aarhus University
3. Denmark
4. Interdisciplinary Nanoscience Center (iNANO)
5. Department of Pathology
6. Aarhus University Hospital
Abstract
Human DNA topoisomerase I (hTopI) is a nuclear enzyme that catalyzes relaxation of super helical tension that arises in the genome during essential DNA metabolic processes.
Publisher
Royal Society of Chemistry (RSC)
Subject
General Materials Science
Link
http://pubs.rsc.org/en/content/articlepdf/2015/NR/C5NR01474C
Reference37 articles.
1. Real-time detection of TDP1 activity using a fluorophore–quencher coupled DNA-biosensor
2. DNA-Based Sensor for Real-Time Measurement of the Enzymatic Activity of Human Topoisomerase I
3. Quantum dot based DNA nanosensors for amplification-free detection of human topoisomerase I
4. Direct measurement of DNA bending by type IIA topoisomerases: implications for non-equilibrium topology simplification
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