Production of a monoclonal antibody for the detection of vitamin B1 and its use in an indirect enzyme-linked immunosorbent assay and immunochromatographic strip-Reference-Cited by-同舟云学术

Production of a monoclonal antibody for the detection of vitamin B1 and its use in an indirect enzyme-linked immunosorbent assay and immunochromatographic strip

Published:2020 Issue:9 Volume:8 Page:1935-1943
ISSN:2050-750X
Container-title:Journal of Materials Chemistry B
language:en
Short-container-title:J. Mater. Chem. B

Author:

Zeng Lu¹²³⁴⁵,Wu Xaioling¹²³⁴⁵,Liu Liqiang¹²³⁴⁵,Xu Liguang¹²³⁴⁵,Kuang Hua¹²³⁴⁵^ORCID,Xu Chuanlai¹²³⁴⁵^ORCID

Affiliation:

1. State Key Laboratory of Food Science and Technology

2. Jiangnan University

3. Wuxi

4. People's Republic of China

5. International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology

Abstract

A monoclonal antibody (mAb) against vitamin B₁ was prepared and based on this, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold-based immunochromatographic test (ICT) strip were developed.

Funder

Ministry of Science and Technology of the People's Republic of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering,General Chemistry,General Medicine

Link

http://pubs.rsc.org/en/content/articlepdf/2020/TB/C9TB02839K

Reference36 articles.

1. Study on interactions of vitamin B1 with sodium dodecyl sulfate for potential food applications: Conductometric, volumetric, calorimetric and spectroscopic approach

2. A novel method for determining vitamin B1 in a wide variety of foodstuffs with or without polyphenols

3. Simple and visual approach for highly selective biosensing of vitamin B1 based on glutathione coated silver nanoparticles as a colorimetric probe