A tunable filter for high molecular weight DNA selection and linked-read sequencing-Reference-Cited by-同舟云学术

A tunable filter for high molecular weight DNA selection and linked-read sequencing

Published:2020 Issue:1 Volume:20 Page:175-184
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Milon Nicolas¹²³⁴⁵,Fuentes Rojas Juan-Luis¹²³,Castinel Adrien⁶⁷⁸⁹³,Bigot Laurent¹⁰¹¹¹²¹³³,Bouwmans Géraud¹⁰¹¹¹²¹³³,Baudelle Karen¹⁰¹¹¹²¹³³,Boutonnet Audrey⁴⁵³,Gibert Audrey⁶⁷⁸⁹³,Bouchez Olivier⁶⁷⁸⁹³,Donnadieu Cécile⁶⁷⁸⁹³,Ginot Frédéric⁴⁵³,Bancaud Aurélien¹²³^ORCID

Affiliation:

1. CNRS, LAAS

2. Toulouse

3. France

4. Adelis Technologies

5. 31670 Labège

6. INRA

7. US 1426 GeT-PlaGe

8. INRA Auzeville

9. Castanet-Tolosan Cedex

10. Univ. Lille

11. CNRS

12. UMR 8523 - PhLAM - Physique des Lasers Atomes et Molécules

13. F-59000 Lille

Abstract

In third generation sequencing, long DNA molecules of more than ∼20 kbp are needed to obtain quality sequence data. Here we report a versatile technology for DNA size selection that fulfills this requirement.

Funder

Agence Nationale de la Recherche

Conseil Régional Midi-Pyrénées

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2020/LC/C9LC00965E

Reference33 articles.

1. Resolving the complexity of the human genome using single-molecule sequencing

2. Reconstructing complex regions of genomes using long-read sequencing technology