New method for detection of T4 polynucleotide kinase phosphatase activity through isothermal EXPonential amplification reaction
Author:
Affiliation:
1. Center for Molecular Recognition and Biosensing
2. School of Life Sciences
3. Shanghai University
4. Shanghai 200444
5. P. R. China
6. Shenzhen Shineway Hi-Tech Co.
7. Ltd
Abstract
A new method has been developed for the sensitive detection of T4 PNKP activity based on the isothermal EXPonential amplification reaction.
Funder
National Natural Science Foundation of China
Publisher
Royal Society of Chemistry (RSC)
Subject
Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2019/AN/C8AN02368A
Reference31 articles.
1. Label-free colorimetric assay for T4 polynucleotide kinase/phosphatase activity and its inhibitors based on G-quadruplex/hemin DNAzyme
2. Repair of DNA Strand Breaks by the Overlapping Functions of Lesion-Specific and Non-Lesion-Specific DNA 3′ Phosphatases
3. Persistent 3′-phosphate termini and increased cytotoxicity of radiomimetic DNA double-strand breaks in cells lacking polynucleotide kinase/phosphatase despite presence of an alternative 3′-phosphatase
4. 3'-Phosphatase activity in T4 polynucleotide kinase
5. Development of a High-Throughput Screening Platform for DNA 3′-Phosphatases and Their Inhibitors Based on a Universal Molecular Beacon and Quantitative Real-time PCR
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