Quantitative and specific detection of viable pathogens on a portable microfluidic chip system by combining improved propidium monoazide (PMAxx) and loop-mediated isothermal amplification (LAMP)-Reference-Cited by-同舟云学术

Quantitative and specific detection of viable pathogens on a portable microfluidic chip system by combining improved propidium monoazide (PMAxx) and loop-mediated isothermal amplification (LAMP)

Published:2021 Issue:32 Volume:13 Page:3569-3576
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Lin Xue¹,Jin Xiangyu¹,Du Wenli¹,Shan Xiaohui¹,Huang Qin¹,Fu Rongxin¹,Lv Wenqi¹,Yang Han¹,Su Ya¹,Huang Guoliang¹^ORCID

Affiliation:

1. Department of Biomedical Engineering, The School of Medicine, Tsinghua University, Beijing 100084, P. R. China

Abstract

Improved propidium monoazide (PMAxx) and loop-mediated isothermal amplification (LAMP) were used to distinguish between viable and dead C. albicans. A portable microfluidic chip system was developed to detect multiple viable pathogens in parallel.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AY/D1AY00953B

Reference32 articles.

1. Microsatellite analysis of genotype distribution patterns of Candida albicans vulvovaginal candidiasis in Nanjing, China and its association with pregnancy, age and clinical presentation

2. Exoenzyme activity and possibility identification of Candida dubliniensis among Candida albicans species isolated from vaginal candidiasis

3. Clinical Framework and Medical Countermeasure Use During an Anthrax Mass-Casualty Incident

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