Affiblot: a dot blot-based screening device for selection of reliable antibodies-Reference-Cited by-同舟云学术

Affiblot: a dot blot-based screening device for selection of reliable antibodies

Published:2021 Issue:35 Volume:13 Page:3874-3884
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Svobodova Zuzana¹²^ORCID,Novotny Jakub¹³^ORCID,Ospalkova Barbora¹,Slovakova Marcela¹^ORCID,Bilkova Zuzana¹^ORCID,Foret Frantisek³^ORCID

Affiliation:

1. Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentska 573, Pardubice, Czech Republic

2. Department of Biological and Medical Sciences, Faculty of Pharmacy in Hradec Kralove, Charles University, Zborovska 2089, Czech Republic

3. Institute of Analytical Chemistry of the CAS, v. v. i., Veveri 967/97, Brno, Czech Republic

Abstract

A report on a palm-sized dot blot-based device that allows simultaneous semi-quantitative comparison of up to five antibodies from different suppliers regarding their affinity/avidity, cross-reactivity, and batch-to-batch reliability.

Funder

Akademie Věd České Republiky

European Regional Development Fund

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AY/D1AY00955A

Reference34 articles.

1. Reproducibility crisis: Blame it on the antibodies

2. Reproducibility: Standardize antibodies used in research